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SKU:54090901
ACTaq High Fidelity DNA Polymerase, originating from Pyrococcus furiosus with molecular weight of 90KDa, is a thermo stable enzyme and exhibits the lower error rate of DNA amplification. For use in DNA fragment extension amplification reactions with 3' to 5' exonuclease proofreading activity; at extension amplification speed of 1000 bases per minute and optimized between 65-75oC, when using dNTP concentration at 100-300µM, Mg2+ concentration at 2.0-3.0mM, and pH at 8.1-9.1. ACTaq High Fidelity DNA Polymerase generates blunt-ended fragment.
Storage Condition -20°C. Stable for at least 12 months in constant freezer temperate
Concentration 5 Units/μL
Unit Definition One unit of the amount of enzyme that incorporates 10nmols of dNTPs into acid-insoluble material in 30 minutes at 72°C
Country of origin United States